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Paul Copeland, PhD

Associate Professor
Office Telephone: 732-235-4670
Lab Telephone: 732-235-5875
paul.copeland@rutgers.edu

Location
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway
Research Tower Room R738
Lab Room 724 & 720

 

Publications

Click Here for PubMed Link to Publications

 

Lab Staff

Louise DeMong Sr. Lab Tech
Nirupama Gupta Postdoctoral Fellow
Sumangala Shetty Postdoctoral Fellow
Aditi Dubey Graduate Student

 

Research Interests

Regulation of gene expression at the translational level, incorporation and utilization of selenocysteine.

Our primary research question targets the protein synthetic machinery as one of the primary sites for the regulation of gene expression and an important sensor of the status of cellular metabolite concentrations including trace elements. The utilization of selenium exemplifies this relationship, and is required for the synthesis and function of an essential group of proteins that contain the amino acid selenocysteine (Sec). In fact, many selenoproteins are known to provide protection from cellular damage and transformation, thus making the synthesis and regulation of these proteins an essential area of research. Sec is incorporated into these proteins by a translational recoding event at specific Stop (UGA) codons that are found upstream of stable stem-loop structures known as Sec insertion sequence (SECIS) elements. While the UGA codon and the SECIS element are the only known cis-acting elements required for Sec incorporation, at least two trans-acting factors are also required: 1) the Sec-specific elongation factor (eEFSec) and 2) a SECIS binding protein (SBP2). One of the ultimate goals for our research is to be able to specifically regulate the expression of potentially beneficial selenoproteins in vivo. In order to achieve this goal, we must understand all of the factors that contribute not only to the basic Sec incorporation reaction but also to the regulation of this process. In addition to characterizing the structure and function of the known factors, much of our work is designed to test hypotheses regarding the identity and function of novel factors involved in the synthesis of selenoproteins utilizing both mammalian systems as well as yeast, a eukaryotic system that is devoid of the Sec incorporation machinery. The results derived from these experiments will not only significantly add to our current knowledge of Sec incorporation, but they will also provide insight into the basic mechanisms of protein synthesis during the elongation and termination phases.