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Thomas J. Kirn, M.D., Ph.D.
Present Title:
Assistant Professor
Department of Pathology & Laboratory Medicine
10/08 to Present

Office Address:
UMDNJ-Robert Wood Johnson Medical School
One Robert Wood Johnson Place – MEB 212    
New Brunswick, NJ 08901

Clinical Expertise:
Clinical Microbiology, Molecular Pathology

Research Expertise:
My general research interests are in the areas of microbial pathogenesis and molecular techniques for infectious disease diagnostics. Current studies include investigation of a novel, rapid method for identification of S. aureus in positive blood cultures and elucidation of molecular mechanisms that contribute to C. difficile pathogenesis.

Undergraduate Education:                                              
Richard Stockton College of New Jersey
Bachelor of Science – Chemistry
09/09-12/95

Graduate Education:
Dartmouth College
Hanover, NH
Doctor of Philosophy, Microbiology and Immunology
09/96-06/03

Dartmouth Medical School
Hanover, NH
Doctor of Medicine
09/96-06/05

Postgraduate Training: 
Hospital of the University of Pennsylvania
Philadelphia, PA
Chief Resident, Clinical Pathology
Department of Pathology and Laboratory Medicine
07/07-10/08
Resident, Clinical Pathology
07/05-10/08

University of Pennsylvania School of Veterinary Medicine
Philadelphia, PA
Postdoctoral Research Fellow, Artis Lab
Department of Pathobiology
07/07-10/08

Academic Appointments:
Assistant Professor
UMDNJ-Robert Wood Johnson Medical School
Department of Pathology and Laboratory Medicine
10/08 to Present

Hospital Appointments:
Medical Staff
Robert Wood Johnson University Hospital
10/08 to Present

Licensure:
State of New Jersey

Professional Organizations:
American Society for Clinical Pathology
College of American Pathologists
American Association for the Advancement of Science
American Society of Microbiology
American Chemical Society

Service on School Committees:
UMDNJ-Robert Wood Johnson Medical School
RWJMS Curriculum Planning Committee; Disease and Defense

Selected Publications:
Refereed Original Articles in Journals:

  • Kirn, T.J. and Keenan, K.A. (1996) Characterization of the distribution of calcium and magnesium
    between the vacuole and cytosol of Neurospora crassa.  Proceedings of the National Council for
    Undergraduate Research, 3(1):1550-1554, 1996
  • Kirn, T.J., Sandoe, C.M.P., Chiang, S.L., Mekalanos, J.J. and Taylor, R.K. (1997) Genetic analysis of TcpA
    pilin structural and functional domains in Vibrio cholerae. Proceedings of the 33rd U.S.-Japan Cholera and Related Diarrheal Diseases Joint Panel Meeting, The United States-Japan Cooperative
    Medical Science Program, December 3-5, 1997
  • Kirn, T.J., Lafferty, M.J., Sandoe, C.M., Taylor, R.K. (2000) Delineation of pilin domains required for bacterial association into microcolonies and intestinal colonization by Vibrio cholerae.  Mol Microbiol; 35(4):896-910
  • Kirn, T.J. and Taylor, R.K.  (2003) Secretion of a soluble colonization factor by the TCP type 4 pilus
    biogenesis pathway in Vibrio cholerae. Mol Microbiol; 49(1): 81-92
  • Hang, L., John, M., Asadazzuman, M., Vanderspurt, C., Bridges, E.A., Qadri, F., Kirn, T.J., Taylor, R.K.,
    Hilman, J.D., Handfied, M., Ryan, E.T., Calderwood, S.B. (2004) Identification of genes uniquely expressed
    during human infection with Vibrio cholerae. Proc Natl Acad Sci USA; 100(14): 8508-8513
  • Taylor, R.K., Kirn, T.J., Meeks, M.D., Wade, T.K. and Wade, W.F. (2004) Vibrio cholerae classical TcpA
    amino acid sequence induces protective antibody that binds an area hypothesized to be important for
    toxin-coregulated pilus structure. Infection and Immunity; 72(10): 6050-6
  • Kirn, T.J. and Taylor, R.K.  (2005) TcpF is a soluble colonization factor and protective antigen secreted by
    El Tor and classical O1 and O139 Vibrio cholerae serogroups. Infection and Immunity; 73(8): 4461-70
  • Kirn, T.J., Jude, B.A. and Taylor, R.K. (2005) A colonization factor that provides a molecular link between
    Vibrio cholerae pathogenesis and environmental survival. Nature; Dec.8: 863-867
  • Kirn, T.J., Levy, N.B., Gosselin, J.J., Rosen, W.J. and Zegans, M.E. (2007) Peripheral T cell lymphoma
    presenting as sclerouveitis. Cornea; Oct: 26(9): 1147-9
  • Krebs, S.J., Kirn, T.J. and Taylor, R.K.  (2009)  Genetic mapping of secretion and functional determinants
    of Vibrio cholerae TcpF colonization factor. Journal of Bacteriology; 191(11): 3665-3776
  • Martinez, R.M., Dharmasena, M.N., Kirn, T.J. and Taylor, R.K.  (2009)  Characterization of two outer
    membrane proteins, FlgO and FlgP that influence Vibrio cholerae motility.  Journal of Bacteriology; doi:10.1128/JB.00632-09 (published ahead of print)
  • Hoffmann, C., Hill, D., Minkah, N., Kirn, T.J., Troy, A., Artis, D. and Bushman, F.  Community-wide
    response of gut microbiota to enteropathogenic Citrobacter infection revealed by deep sequencing.
    Infect Immun doi:10.1128/IAI.00493-09 (published ahead of print)
  • Park JY, Kirn TJ, Artis D, Waldman SA, Kricka LJ. Chemiluminescence-based detection of gastrointestinal malignancies. Luminescence. 2009 Oct 13. [Epub ahead of print] PubMed PMID: 19827005
  • Hill DA, Hoffmann C, Abt MC, Du Y, Kobuley D, Kirn TJ, Bushman FD, Artis D. Metagenomic analyses
    reveal antibiotic-induced temporal and spatial changes in intestinal microbiota with associated alterations in immune cell homeostasis. Mucosal Immunol. 2010 Mar;3(2):148-58. Epub 2009 Nov 25. PubMed PMID: 19940845

    Abstracts:
  • Krebs, S.J., Kirn, T.J. and Taylor R.K.  Characterization of a Secreted Vibrio cholerae Virulence Factor
    Required for Intestinal Colonization.  Poster B-364, ASM General Meeting, Atlanta, GA, June 5-9, 2005
  • Kirn, T.J., Jude, B.A. and Taylor R.K.  GbpA, a GlcNac binding protein important for Vibrio cholerae intestinal colonization and attachment to chitin.  ASM General Meeting, Washington, DC, May 18-22, 2003
  • Hang, L., John, M., Asadazzuman, M., Vanderspurt, C., Bridges, E.A., Qadri, F., Kirn, T.J., Taylor, R.K.,
    Hilman, J.D., Handfied, M., Ryan, E.T., Calderwood, S.B.  Identification of genes uniquely expressed during
    human infection with Vibrio cholerae.  ASM General Meeting, Washington, DC, May 18-22, 2003
  • Krebs, S.J., Kirn, T.J. and Taylor, R.K.  A Secreted Vibrio cholerae Virulence Factor Required for Intestinal Colonization.  ASM General Meeting, Washington, DC, May 18-22, 2003
  • Hang, L., John, M., Asadazzuman, M., Vanderspurt, C., Bridges, E.A., Qadri, F., Kirn, T.J., Taylor, R.K.
    Hilman, J.D., Handfied, M., Ryan, E.T., Calderwood, S.B.  Use of In-vivo-induced antigen technology to
    identify genes uniquely expressed during human infection with Vibrio cholerae. International Centers for
    tropical Diseases Research Network Annual Meeting, Bethesda, MD, May 13-15, 2003
  • Kirn, T.J. and Taylor, R.K.  A secreted colonization factor associated with the Vibrio cholerae toxin
    coregulated pilus.  Microbial Pathogenesis and Host Response, Cold Spring Harbor Laboratory, New York,
    Sept. 22-26, 1999
  • Kirn, T.J., Sandoe, C.M.P. and Taylor, R.K. Genetic analysis of TcpA structure and function.  Microbial
    Pathogenesis and Host Response, Cold Spring Harbor Laboratory, New York, Sept. 10-14, 1997
  • Keenan, K.A., Kirn, T.J. and Wisniewski, T. Characterization of calcium and magnesium uptake in the
    vacuole of Neurospora crassa.  Nineteenth Fungal Genetics Conference, Pacific Grove, CA, March 18-23,
    1997
  • Kirn, T.J. and Keenan, K.A.  Distribution and transport of calcium and magnesium in the vacuole of
    Neurospora crassa. 10th Annual National Conference on Undergraduate Research, University of North
    Carolina, June 10-14, 1996
  • Kirn, T.J. and Keenan, K.A.  Measurement of cellular levels of calcium and magnesium via atomic absorption spectrophotometry. 13th Middle Atlantic Regional Meeting of American Chemical Society, Washington, D.C.,
    May 12-16, 1995
  • Kirn, T.J. and Taylor, R.K.  A secreted colonization factor associated with the Vibrio cholerae toxin
    coregulated pilus.  Gordon Research Conference, Molecular Mechanisms of Microbial Adhesion,
    August 1-6, 1999
  • Kirn, T.J. and Taylor, R.K.  Characterization of colonization factors in Vibrio cholerae.  Gordon Research
    Conference, Microbial Toxins and Pathogenicity, July 12-17, 1998

    Invited Articles:
  • Taylor, R.K., Kirn, T.J., Bose, N., Stonehouse, E., Tripathi, S., Kovac, P., Wade, W.F. (2004) Progress
    towards development of a cholera subunit vaccine.  Chemistry and Biodiversity; 1(7): 1036-1057

Invited Presentations and Lectures:

  • “CURRENT AND EMERGING APPLICATIONS OF MOLECULAR METHODS IN THE CLINICAL LABORATORY”
    American Society for Clinical Laboratory Sciences – New Jersey, Oct. 29 2009, Scotch Plains, NJ