The Flow Cytometry Core Facility (FCCF) of Rutgers Robert Wood Johnson Medical School (RWJMS), provides flow cytometry (a.k.a. “FACS”) and cell sorting services to investigators from Rutgers University, Princeton University and the surrounding academic and commercial communities.
The FCCF currently has an FC-500 flow cytometer (2-laser, 5-color detection), and an ultra-high speed Influx cell sorter (4-laser, 12-colors) with sterile capability and biohazard containment.
The FCCF provides instrumentation and expertise in flow cytometry and cell sorting. Samples are usually prepared by the investigator and brought to the Facility to be run with or without staff assistance. We also collaborate in experimental design, data analysis, and preparation of publications and grants. The services provided by the FCCF are intended to help develop strategies to prevent, diagnose, and treat cancer and other genetic and infectious diseases, and to study the underlying biology of these conditions.
The RWJMS Flow Cytometry Core Facility is a major component of the Flow Cytometry & Cell Sorting Shared Resource of CINJ, which includes three sites:
- Rutgers RWJMS Flow Cytometry Core Facility at CINJ, New Brunswick Campus
- Rutgers Analytical Cytometry/ Image Analysis Core Facility at Rutgers University, Piscataway Campus
- Princeton Flow Cytometry Resource Facility at Princeton University, in Princeton
Administrative Structure of the FCCF
Administrative oversight of the Flow Cytometry Core Facility is provided by the RWJMS Office of the Dean. The FCCF is an Institutional Shared Resources of RWJMS, and is part of the Cancer Institute of New Jersey (CINJ) Flow Cytometry & Cell Sorting Shared Resource. As such, it is operated as a shared service. The facility is supported in part by the Cancer Center Support Grant (CCSG) from the National Cancer Institute. Additional support is provided by Rutgers RWJMS, and other sources, including charge-backs and/or other grants.
Edmund Lattime, PhD, Professor of Surgery, Deputy Director of CINJ, serves as scientific director of the FCCF, who sets priorities for use after consultation with the Advisory Committee. Robert Prodoehl, RWJMS Director of Operations, provides administrative oversight of resource operations pertaining to operating costs, user fees, usage reports and trends, institutional support, and billing. Billing is done through the Priority Software® Facility Billing System (FBS), which provides electronic invoices, usage analysis reporting, charge back recovery/income forecasting, integrated accounts receivable system, and resource scheduling capabilities.
Advisory Committee of the FCCF
The Advisory Committee is comprised of leadership from RWJMS and its Centers of Excellence (Cancer Institute of New Jersey, Child Health Institute of New Jersey, and Stem Cell Institute of New Jersey), NIH/NCI- funded CTSA and CTEP programs, and high-use Academic Departments. Members of the Advisory Committee include institute and program leaders, major and highly-experienced users, and administration (listed alphabetically):
- Linda Barker, Associate Director for Administration and Planning, CINJ
- Joseph R. Bertino, M.D., Chief Scientific Officer, CINJ
- Edmund Lattime, Ph.D., Professor of Surgery, Associate Director for Education and Training, CINJ
- Arnold B. Rabson, M.D., Director, Child Health Institute of New Jersey, RWJMS
- Kathleen W. Scotto, Ph.D., Dean, Graduate School of Biological Sciences, RWJMS
- Yufang Shi, Ph.D. D.V.M., Professor, Child Health Institute of New Jersey, RWJMS