Laboratory Policies

Facility Acknowledgement in Publications, Papers, and Grant Applications

We request that users formally acknowledge the Flow Cytometry Core Facility in their research. We depend on funding from sources that expect to see the Core Facility's contribution to successful research efforts, and this is  best demonstrated through recognition in published papers and grant applications. Therefore, we request that any publications, papers, grant applications, or other presentations of research results that utilize(d) the resources of our Facility include a formal acknowledgment. We suggest the following language:

This research was supported by the Flow Cytometry Core Facility of Rutgers Robert Wood Johnson Medical School, a Shared Resource of The Rutgers Cancer Institute of New Jersey (P30CA072720) and NIH Shared Instrumentation Grant (1 S10 RR025468-01).

We also ask that you consider acknowledging individuals from the Facility by name if they have provided any intellectual input or special efforts in your studies.

Samples for Analysis

Samples for analysis or sorting are normally prepared by investigators in their own laboratories using their own reagents and equipment. These samples are then brought to the Core Facility, along with a completed sample form, including biosafety and billing information. Core facility personnel can advise investigators regarding optimal sample preparation, and provide copies of standard procedures and relevant references to assist in the development of techniques. Investigators new to the methods of sample preparation for flow cytometry should consult with the Facility Manager regarding sample preparation protocols and the need for proper control samples.

All investigators should consult with the Facility Manager concerning operating policies, and to discuss optimization of experimental design, sample preparation, appropriate controls, as well as biosafety issues in the case of sorting of any biohazardous samples. Any questions regarding handling of live samples from humans or primates should be directed to the Facility Manager, well in advance of the actual experiment.  Samples containing any type of radioactive material are not accepted.

Details on sample preparation requirements are available here.

Reservations and appointments

FC-500 Flow Cytometer
Reservations for the FC 500 should be made on the sign-up sheet. Please reserve the actual time that you expect to use the instrument, not including start-up or shut-down time. The minimum reservation time is 15 minutes, with 15-minute billing increments. 

Influx Cell Sorter
Requests for cell sorting appointments should be made directly to the Facility Manager via email: or telephone: 732-235-8384.  Cell sorting is conducted by Core personnel only; there is no self-service.  The minimum charge time for cell sorter usage is 1 hour, with additional 15-minute increments. 

Please cancel as early as possible if you will not be using your reserved time.  Idle time on the machines is a costly waste that we would like to avoid.  Accordingly, cancellations made at least 24 hours in advance will not incur a fee. Cancellations made less than 24 hours in advance will be billed for the scheduled time, or for a minimum of 1 hour, at the discretion of the Facility Manager. All cancellations should be communicated directly to the Facility Manager, by email or telephone 732-235-8384.

Training for FC 500 Cytometer Users
To use the FC 500 analytical flow cytometer, all users must undergo training before using the instrument on their own. Individual training is provided by the Flow Cytometry Core facility. The cost of training is billed at the same rate as assisted usage.

Data Downloads and Archives

Please see our policy on Data Downloading and Archives.

Data Downloads and Archiving

Data files are automatically stored on the computer workstations. For the FC-500, data is automatically stored as both raw data (listmode files with .LMD suffix) and a PDF of any "flowpage" layout created by the software. To prevent the hard drive from filling up, all raw data files should be downloaded by individual users within 6 months.  Files may be copied to USB flash drive (we will loan you ours), CD, DVD, or 3.5 inch diskettes (not provided). Users should download their files routinely, preferably after each use, to avoid possible data loss. The Facility manager will make every attempt to create a long-term backup copy of all data, however this cannot be guaranteed.

WARNING: Any data files remaining after more than 24 months on the computer workstations will be permanently deleted and may become unavailable.