Links to Resources
The following links to relevant information resources are provided for your convenience.
Instrument Information
FC 500 Flow Cytometer
Influx Cell Sorter
Software Information
FlowJo Analysis Software
FlowJo is a premier flow cytometry analysis software package (see www.flowjo.com). It can analyze data from almost any flow cytometer or cell sorter platform, including BD, Beckman-Coulter, and Millipore instruments, and is available for Windows of Mac computers. A free 30-day demo version is available without obligation: FlowJo Demo Download
Rutgers-RBHS has a multi-user site license, which allows users to run FlowJo on their own computers for a discounted price. Each license is for use on a single, designated computer, either Windows or Mac; however, it may be transferred to a different computer if necessary. Only users with a Rutgers email address qualify. Other users should inquire with the Facility manager.
For current pricing of FlowJo user licenses, see FCCF Price list. The license is valid for 1 year, renewable annually. This is a cooperative arrangement with the Flow Cytometry Core Laboratory of NJMS; cost will be billed via interdepartmental transfer.
For a license request form, click here. Please complete the first two sections (contact information and billing information; no need to fill out the Project section). Completed forms should be faxed to 973-972-7507 on the Newark campus. For inquiries regarding the FlowJo site license, please contact the site license administrator by phone: 973-972-7502, or email: singhs1@rutgers.edu.
General Tools for Flow Cytometry
- Fluorescence Spectrum Viewer from BD Biosciences is a a multi-color flow cytometry design tool. It allows the proper selection of fluorescent dyes, lasers, and optical filters for your experiment.
- Current Protocols in Flow Cytometry provides comprehensive information on theory, techniques, and applications. This searchable reference is a good place to start.
- Comprehensive information on flow cytometric analysis is available from Instrument manufacturers:
- BD Biosciences Multicolcor Flow Cytometry Tools has information on Absorption and Emission Spectra, Fluorochrome Specifications, Multicolor Panel Design, Application Notes, and Examples of Human and Mouse Antibody Panels.
- Beckman Coulter Flow Cytometry Info is a compehensive resource for flow cytometry products, instrumetns, and techniques. This site also has a searchable and downloadable version of an excellent reference book by Howard M. Shapiro: Practical Flow Cytometry, 4th ed.
